Post-Translational Modifications Analysis
Post-Translational Modifications Analysis
Proteins are subjected to a wide variety of covalent modifications after translation. Many of these post-translational modifications (PTMs) are critical to the protein’s function. The common PTMs include Phosphorylation, Ubiquitination, Methylation, Acetylation and Glycosylation. We have accumulated experiences to develop reliable workflows using available tools and technologies for identifications of PTMs.
+ P301: PTM Identification
Identification of Post-Translational Modifications
Price: $600 per sample
Turnaround Time: 10 to 15 business days
Service Description
• Identification of PTMs (S/T/Y phosphorylation, R/K methylation, K/H acetylation, and K ubiquitination sites) for a target protein from purified protein samples or partially purified protein mixture samples
• For identification of specific PTMs, please contact us for a quote
• Workflow: Sample preparation, enzymatic digestion, LC-MS/MS analysis, data analysis, and results report
Deliverables
• PTM Identification Report
• List of identified proteins
• All identified peptides sequences with possible PTMs for target protein(s)
• The target protein’s PTMs spectra
• Raw data files, upon request
Suitable Sample Type
• Purified protein, in solution or dried
• Purified protein complex
• Protein mixture from pull-down, immunoprecipitation (IP), co-immunoprecipitation (co-IP), or other approaches
• At least 0.5 µg of target protein required
• Identification of PTMs (S/T/Y phosphorylation, R/K methylation, K/H acetylation, and K ubiquitination sites) for a target protein from purified protein samples or partially purified protein mixture samples
• For identification of specific PTMs, please contact us for a quote
• Workflow: Sample preparation, enzymatic digestion, LC-MS/MS analysis, data analysis, and results report
• PTM Identification Report
• List of identified proteins
• All identified peptides sequences with possible PTMs for target protein(s)
• The target protein’s PTMs spectra
• Raw data files, upon request
• Purified protein, in solution or dried
• Purified protein complex
• Protein mixture from pull-down, immunoprecipitation (IP), co-immunoprecipitation (co-IP), or other approaches
• At least 0.5 µg of target protein required
+ P302: Phosphoprotein Profiling
Profiling of S/T/Y Phosphorylation Sites Using a Phosphopeptides Enrichment Approach and Label-Free Quantification Method for Comparison Among Different Samples
Price: $2,000 per sample
Turnaround Time: 15 to 20 business days
Service Description
• Profiling of S/T/Y phosphorylation sites at global level from treated cell pellets (> 2×107 cell/sample), fresh frozen tissues (> 500 mg/sample), or fresh frozen total cell or tissue lysates (200 µl at 5 mg/ml)
• For profiling of specific PTMs, please contact us for a quote
• Can be tailored for quantitative profiling and comparison between two samples, or between one control and several different treatments
• Up to 1,000 phosphorylation sites identified
• Workflow: Sample preparation, enzymatic digestion, phosphopeptides enrichment using TiO2 beads, LC-MS/MS analysis, data analysis, and results report
Deliverables
• PTM Identification Report
• List of identified proteins
• All identified peptides sequences with phosphorylation sites
• Label-free quantification
• Raw data files, upon request
Suitable Sample Type
• Fresh frozen cell pellet (≥ 20 million cells per sample recommended for optimal results)
• Fresh frozen tissue (≥ 500 mg recommended for optimal results)
• Total cell or tissue lysate (≥ 500 μg of total protein recommended for optimal results)
• Profiling of S/T/Y phosphorylation sites at global level from treated cell pellets (> 2×107 cell/sample), fresh frozen tissues (> 500 mg/sample), or fresh frozen total cell or tissue lysates (200 µl at 5 mg/ml)
• For profiling of specific PTMs, please contact us for a quote
• Can be tailored for quantitative profiling and comparison between two samples, or between one control and several different treatments
• Up to 1,000 phosphorylation sites identified
• Workflow: Sample preparation, enzymatic digestion, phosphopeptides enrichment using TiO2 beads, LC-MS/MS analysis, data analysis, and results report
• PTM Identification Report
• List of identified proteins
• All identified peptides sequences with phosphorylation sites
• Label-free quantification
• Raw data files, upon request
• Fresh frozen cell pellet (≥ 20 million cells per sample recommended for optimal results)
• Fresh frozen tissue (≥ 500 mg recommended for optimal results)
• Total cell or tissue lysate (≥ 500 μg of total protein recommended for optimal results)
+ P303: Tandem Mass Tag Labeling Quantitative Phosphoprotein Profiling
Quantitative Comparison of the Phosphoproteomes of 6-18 Samples in One Set of Analysis Using Thermo Scientific TMTpro™ 18-Plex Label Reagent Set and Phosphopeptides Enrichment Kit
Price: $1,200 per sample
Turnaround Time: 20 to 25 business days
Service Description
• Quantitative profiling of phosphoproteins at global level for up to 18 samples in one set of analysis using Thermo Scientific TMTpro™ 18-Plex Label Reagent Set and Phosphopeptides Enrichment Kit
• Recommended for analysis of samples in triplicates
• Up to 1,000 phosphorylation sites identified at 1% FDR
• Pathway analysis of identified differential phosphoproteins using PROMICPATH
• Workflow: Protein lysate preparation, enzymatic digestion, TMT-labeling, UHPLC fractionation, TiO2 Phosphopeptides enrichment, LC-MS/MS analysis, TMT quantitation, data analysis, and results report
Deliverables
• Proteome Profiling Report
• List of identified proteins with TMT labeling based relative abundance
• List of all identified proteins with possible phosphorylation sites and matched peptides sequences
• List of proteins with phosphorylation that are differentially expressed among samples (e.g., > 2 folds)
• Data normalization, statistical analysis, volcano plots, heat maps
• Data figures
• Raw data files, upon request
Suitable Sample Type
• Fresh frozen cell pellet (≥ 10 million cells per sample recommended for optimal results)
• Fresh frozen tissue (≥ 500 mg recommended for optimal results)
• Total cell or tissue lysate (≥ 500 μg of total protein per sample is recommended for optimal results)
• Quantitative profiling of phosphoproteins at global level for up to 18 samples in one set of analysis using Thermo Scientific TMTpro™ 18-Plex Label Reagent Set and Phosphopeptides Enrichment Kit
• Recommended for analysis of samples in triplicates
• Up to 1,000 phosphorylation sites identified at 1% FDR
• Pathway analysis of identified differential phosphoproteins using PROMICPATH
• Workflow: Protein lysate preparation, enzymatic digestion, TMT-labeling, UHPLC fractionation, TiO2 Phosphopeptides enrichment, LC-MS/MS analysis, TMT quantitation, data analysis, and results report
• Proteome Profiling Report
• List of identified proteins with TMT labeling based relative abundance
• List of all identified proteins with possible phosphorylation sites and matched peptides sequences
• List of proteins with phosphorylation that are differentially expressed among samples (e.g., > 2 folds)
• Data normalization, statistical analysis, volcano plots, heat maps
• Data figures
• Raw data files, upon request
• Fresh frozen cell pellet (≥ 10 million cells per sample recommended for optimal results)
• Fresh frozen tissue (≥ 500 mg recommended for optimal results)
• Total cell or tissue lysate (≥ 500 μg of total protein per sample is recommended for optimal results)
submission Guidelines
