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P601: Evaluation Of Identity And Purity

Evaluation of Purified or Partially Purified Protein Samples Including Therapeutics Products at the Early Stage of Development

Price: $600 per sample

Turnaround Time: 5 to 10 business days

Service Description:

  • Identification by enzymatic digestion followed by LC-MS/MS analysis
  • All detectable proteins (even at ng level) including any host proteins are identified and their relative abundance determined
  • Purity is estimated based on the relative abundance of identified proteins
  • Workflow: Sample preparation, enzymatic digestion, LC-MS/MS analysis, data analysis, and results report

Deliverables:

  • Protein Analysis Report
  • List of identified proteins (target and host proteins)
  • List of identified proteins with matched peptides sequences
  • Purity
  • Raw data files, upon request

Suitable Sample Type:

  • Purified protein samples, in solution or dried (> 5 µg/sample)
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P602: Full Amino Acid Sequence Confirmation

Confirmation of Full Amino Acid Sequence (>95% Sequence Coverage) of Any Purified Protein Sample With a Known Target Protein Amino Acid Sequence

Price: $1,200 per sample (for de novo sequencing, please contact us for a quote)

Turnaround Time: 10 to 15 business days

Service Description:

  • Confirmation of full amino acid sequence (> 95% sequence coverage) of purified protein samples (purity > 80%)
  • Additional charge applies for mAbs, as both heavy chain and light chain sequences are confirmed
  • All detectable proteins (even at ng level) including any host proteins are identified and their relative abundance determined
  • Purity is estimated based on the relative abundance of identified proteins
  • Workflow: Sample preparation, enzymatic digestion, LC-MS/MS analysis, data analysis, and results report

Deliverables:

  • Protein Analysis Report
  • List of peptides identified for target protein and peptide mapping
  • List of identified host proteins, if any
  • List of identified host proteins with matched peptides sequences
  • Purity
  • Raw data files, upon request

Suitable Sample Type:

  • Purified protein samples, in solution or dried (> 20 µg/sample; purity > 80% )
Add To Cart Request Quote

P603: Identification Of N-Linked Glycosylation Sites

Identification of N-Linked Glycosylation Sites From Purified Protein Samples

Price: $1800 per sample (for O-linked glycosylation, please contact us for a quote)

Turnaround Time: 10 to 15 business days

Service Description:

  • Identification of N-linked glycosylation sites and confirmation of full amino acid sequence (> 95% sequence coverage) of purified protein samples (e.g., mAb (purity > 95%) using deglycosylation treatment and LC-MS/MS
  • Quantification of glycosylation is determined using label-free quantitation method
  • All detectable proteins (even at ng level) including any host proteins are identified and their relative abundance determined
  • Purity is estimated based on the relative abundance of identified proteins
  • Workflow: Sample preparation, deglycosylation treatment, enzymatic digestion, LC-MS/MS analysis, data analysis, and results report

Deliverables:

  • Protein Analysis Report
  • N-linked glycosylation sites
  • List of Peptides identified for target protein and peptide mapping
  • List of identified host proteins, if any
  • List of identified host proteins with matched peptides sequences
  • Purity
  • Raw data files, upon request

Suitable Sample Type:

  • Purified protein samples, in solution or dried (> 20 µg/sample; purity > 99%)
  • If the purity is less than 90%, please contact us for a quote
Add To Cart Request Quote

P604: Profile Of N-Linked Glycans For Monoclonal Antibodies

Characterization of the Profile of N-Linked Glycans Released From Purified mAb Samples Using Rapifluor-Ms™ N-Glycan Kit and LC-MS/MS

Price: $5000 per sample

Turnaround Time: 20 to 35 business days

Service Description:

  • Profiling of N-linked glycans released from pure mAbs (note: If multiple N-linked glycosylation sites are identified, the N-linked glycans profiling analysis will not be performed)
  • Relative abundance of glycans is determined based on label-free quantitation method (Ion intensity/peak area)
  • Workflow: Sample preparation, deglycosylation treatment, enzymatic digestion, N-linked glycans labeling, LC-MS/MS analysis, data analysis, and results report

Deliverables:

  • Protein Analysis Report
  • N-linked glycans profile
  • N-linked glycosylation sites
  • List of peptides identified for target protein and peptide mapping
  • List of identified host proteins, if any
  • List of identified host proteins with matched peptides sequences
  • Purity
  • Raw data files, upon request

Suitable Sample Type:

  • Purified protein samples, in solution or dried (> 50 µg/sample; purity > 99% )
Add To Cart Request Quote

Sample Preparation Guidelines

Sample Preparation Guidelines
Therapeutics Characterization

High-Resolution Characterization of Therapeutic Proteins

Poochon developed a robust approach using a range of state-of- the-art orthogonal methodologies to elucidate the primary amino acid sequence, post-translational modifications, and associated micro-heterogeneity and glycosylation of therapeutic products.

Learn More About Our Technology

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