PCR Genotyping
Genotyping
Genotyping is an invaluable tool to identify organisms carrying genetic variations, including insertions and deletions. This method involves the extraction of DNA from animal tissue samples and the subsequent amplification of genomic regions of interest by polymerase chain reaction (PCR). The amplified products are then analyzed based on their size by agarose gel electrophoresis, with an option to confirm the expected sequence by Sanger DNA sequencing
Poochon’s rapid mouse genotyping services are fully customizable, including custom assay development options and testing of multiple tissue types. Through many years of Sanger DNA sequencing collaboration with Quintara Biosciences and our 96-capillary 3730xl DNA Analyzers, the gold standard for high throughput genetic analysis, we have the capability to run multiple types of allele-specific assays, including PCR, DNA sequencing, and single SNP assay.
+ G101: PCR Genotyping
PCR Genotyping
Price: $6.50 per sample (if more than 1 PCR reaction is required, an additional charge of $2.50 will be assessed)
Turnaround Time: 2 to 3 business days
Service Description: Genomic DNA isolation, PCR, PCR fragment electrophoresis
Deliverables: DNA gel images, DNA sequencing results, genotyping report
Suitable Sample Type: Mouse tail/ear, or genomic DNA extraction
+ G102: PCR + Sequencing Genotyping
PCR + Sequencing Genotyping
Price: $15.00 per sample (if more than 1 PCR reaction with sequencing is required, a charge of $11.00 per additional reaction will be assessed)
Turnaround Time: 2 to 3 business days
Service Description:
Genomic DNA isolation, PCR, PCR fragment electrophoresis, DNA sequencing (target gene ≤800 bp)
Deliverables: DNA gel images, DNA sequencing results, genotyping report
Suitable Sample Type: Mouse tail/ear, or genomic DNA extraction
+ G103: New Mouse Line Genotyping Analysis Setup
New Mouse Line Genotyping Analysis Setup
Price: $300.00
Turnaround Time: 3 to 5 business days
Service Description: Genomic DNA isolation, PCR, PCR fragment electrophoresis, PCR validation (target gene ≤1500 bp)
Deliverables: DNA gel images, genotyping report
Suitable Sample Type: Mouse tail/ear, or genomic DNA extraction
submission Guidelines
